There are two cycle hire locations at the Southerly end of the Manifold Trail.
Brown End Farm Cycle Hire
http://www.manifoldcycling-brownendfarm.co.uk/ch_main.html
The Manifold Track Cycle Hire Centre
http://www.visitpeakdistrict.com/Leek-Manifold-Valley-Cycle-Hire/details/?dms=3&venue=6026075